<i>GmTNRP1</i>, associated with rhizobial type‐III effector NopT, regulates nitrogenase activity in the nodules of soybean (<i>Glycine max</i>)

Soybean is a rich source of protein and oil for humans. It forms symbiotic association with rhizobia to fix atmospheric nitrogen its growth development. Rhizobial type-III effectors act as important signaling molecules in symbiosis. However, they are poorly studied. In this study, mutation TtsI Sinorhizobium fredii HH103 reduced nodule numbers dry weights SN14 (cultivated soybean) ZYD00006 (wild soybean). Moreover, it nitrogenase activity but not ZYD00006. Based on the differences between inoculated mutant, two conditional quantitative trait loci associated were identified chromosome segment substitution lines derived from Using transcriptome analysis, single-nucleotide polymorphism (SNP) qRT-PCR, Glyma.13G150000 (GmTNRP1) was candidate gene soybean that activity, along NopT (a effector). addition, GmTNRP1 (an LRR-RK family located cell membrane) observed be induced by negatively regulate activity. transgenic hairy roots silencing, no significant difference terms nodules mutant those type). decreased after overexpression GmTNRP1. SNP promoter affected expression response NopT. Haplotype analysis revealed belonged Hap1 Hap2, which explained different results varieties. To best our knowledge, first study identify rhizobial effector nodules. can This enhanced understanding mechanism underlying rhizobia.